Analyses of binding sequences of the PhaR protein of Rhodobacter sphaeroides FJ1

Autor:	Mei-Kwei Yang, Min-En Chou
Rok vydání:	2010
Předmět:	chemistry.chemical_classification biology Binding protein Plasma protein binding biology.organism_classification Microbiology Rhodobacter sphaeroides Biochemistry chemistry Genetics Nucleotide Binding site Molecular Biology Rhodospirillaceae Gene Palindromic sequence
Zdroj:	FEMS Microbiology Letters. 302:138-143
ISSN:	1574-6968 0378-1097
DOI:	10.1111/j.1574-6968.2009.01836.x
Popis:	The phaC, phaP, phaR, and phaZ genes are involved in the synthesis, accumulation, and degradation of poly-beta-hydroxybutyrate (PHB). These genes encode the PHB synthase, phasin, regulatory protein, and PHB depolymerase, respectively, and are located in the same locus in the genome of Rhodobacter sphaeroides FJ1, a purple nonsulfur bacterium capable of producing PHB. We have previously found that the PhaR protein binds to the promoter regions of phaP, phaR, and phaZ and represses their expression. In this study, we determined that PhaR binds to an 11-bp palindromic sequence, 5'-CTGCN(3)GCAG-3', located at nucleotides -69 to -59 and -97 to -87 relative to the translation start site of phaP. Substitution of the three spacer nucleotides with any three or four nucleotides in this sequence had no effect on PhaR binding, but all other base deletions or substitutions in this sequence abolished its ability to bind PhaR both in vitro and in vivo. These results suggest that PhaR regulates the expression of phaP in R. sphaeroides FJ1.
Databáze:	OpenAIRE
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