| Popis: |
Corynebacterium glutamicum and a few closely related species have long occupied a central role in the production of amino acids and other primary metabolites by fermentation. Recombinant DNA technology provides a powerful array of genetic tools for the manipulation of gene expression in this species. The cloning of genes in the threonine biosynthetic pathway will be discussed, with emphasis on the identification of sequences responsible for the expression and regulation of the homthrB operon. Recent experiments, utilizing site-directed mutagenesis, demonstrate that these genes are controlled by a single stem-loop attenuator containing a methionine leader residue. |
