Alkaline protease production by halophilic Bacillus sp. strain SP II-4 and characterization with special reference to contact lens cleansing
| Autor: | R.P. Rejisha, M. Murugan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
010302 applied physics
chemistry.chemical_classification Protease food.ingredient Strain (chemistry) medicine.medical_treatment Disinfectant 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Halophile Contact lens Agar plate food Enzyme chemistry 0103 physical sciences Skimmed milk medicine Food science 0210 nano-technology |
| Zdroj: | Materials Today: Proceedings. 45:1757-1760 |
| ISSN: | 2214-7853 |
| DOI: | 10.1016/j.matpr.2020.08.624 |
| Popis: | This study aims to screening of protease producing bacterial strain from saltpan located in Kanyakumari. Proteolytic bacterial strains were isolated by using skim milk agar plates. The potent bacterial isolate (SP II-4) was typically identified as Bacillus sp. SP II-4 by studying phenotypic characteristics. The purified enzyme fraction showed 591.04 (U/mg) of specific activity. The protease enzyme liberated from this strain illustrated better stability and activity in pH 10.0, 40 °C and stable upto 7.5% salt. The protease enzyme demonstrated better content lens cleansing effectiveness along with common chemical disinfectant. These above properties of the enzyme clearly stating that may use in various industrial processes which are performed even at extreme conditions. |
| Databáze: | OpenAIRE |
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