Bacterial diversity shift determined by different diets in the gut of the spotted wing flyDrosophila suzukiiis primarily reflected on acetic acid bacteria
| Autor: | Elena Gonella, Violetta Vacchini, Bessem Chouaia, Fabio Mazzetto, Alberto Alma, Elena Crotti, Francesca Mapelli, Mauro Mandrioli, Daniele Daffonchio, Matteo Callegari, Erica M. Prosdocimi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
education.field_of_study biology media_common.quotation_subject fungi 030106 microbiology Population Zoology Insect Ribosomal RNA Gut flora biology.organism_classification Agricultural and Biological Sciences (miscellaneous) Microbiology 03 medical and health sciences 030104 developmental biology PEST analysis Acetic acid bacteria education Drosophila suzukii Drosophila Ecology Evolution Behavior and Systematics media_common |
| Zdroj: | Environmental Microbiology Reports. 9:91-103 |
| ISSN: | 1758-2229 |
| DOI: | 10.1111/1758-2229.12505 |
| Popis: | The pivotal role of diet in shaping gut microbiota has been evaluated in different animal models, including insects. Drosophila flies harbour an inconstant microbiota among which acetic acid bacteria (AAB) are important components. Here, we investigated the bacterial and AAB components of the invasive pest Drosophila suzukii microbiota, by studying the same insect population separately grown on fruit-based or non-fruit artificial diet. AAB were highly prevalent in the gut under both diets (90 and 92% infection rates with fruits and artificial diet respectively). Fluorescent in situ hybridization and recolonization experiments with green fluorescent protein (Gfp)-labelled strains showed AAB capability to massively colonize insect gut. High-throughput sequencing on 16S rRNA gene indicated that the bacterial microbiota of guts fed with the two diets clustered separately. By excluding AAB-related OTUs from the analysis, insect bacterial communities did not cluster separately according to the diet, suggesting that diet-based diversification of the community is primarily reflected on the AAB component of the community. Diet influenced also AAB alpha-diversity, with separate OTU distributions based on diets. High prevalence, localization and massive recolonization, together with AAB clustering behaviour in relation to diet, suggest an AAB role in the D. suzukii gut response to diet modification. |
| Databáze: | OpenAIRE |
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