Vasopressin Receptors in Human Adrenal Medulla and Pheochromocytoma1
| Autor: | Eric Grazzini, Gilles Guillon, G. Boccara, Pascal Colson, Sylvain Derick, Christophe Breton, Claudine Serradeil-Le Gal, Danièle Raufaste, Miriam Andrés, Nathalie C. Guérineau, Frédérique Rickwaert |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Adrenal cortex Endocrinology Diabetes and Metabolism Biochemistry (medical) Clinical Biochemistry Biology medicine.disease Biochemistry Pheochromocytoma Endocrinology medicine.anatomical_structure Internal medicine medicine Catecholamine Adrenal medulla Receptor Cellular localization Medulla Vasopressin receptor medicine.drug |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 84:2195-2203 |
| ISSN: | 1945-7197 0021-972X |
| Popis: | The nature of vasopressin (VP) receptors present in normal and tumoral human adrenal was investigated using various experimental approaches. Specific VP-binding sites were detected by autoradiography using[ 3H]arginine VP as a radioligand in adrenal cortex and medulla. The V1a receptor subtype was expressed in the two parts of the gland, as shown by pharmacological studies and RT-PCR experiments. By contrast, the V1b receptor subtype was only expressed in medullary chromaffin cells. This was confirmed by the characterization of V1b transcripts detected in adrenal medulla tissues. In pheochromocytoma, we also detected functional V1b receptors. These receptors triggered intracellular calcium mobilization from intracellular pools and were involved in catecholamine secretion. Binding experiments performed on pheochromocytoma plasma membrane preparations also revealed V1a vasopressin-binding sites, whose roles and cellular localization have not yet been determined. RT-PCR experiments confirmed these data; 100%... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|