Molecular phylogeny of the Pycnonotus sinensis and Pycnonotus taivanus in Taiwan based on sequence variations of nuclear CHD and mitochondrial cytochrome b genes

Autor: Yii-Cheng Chou, Chun-An Cheng, Ying-Fang Su, Chien-Chung Cheng, Hsueh-Wei Chang, Hsien-Chung Lee, Chi-Li Tsai, Cheng-Hao Wen, Cheng-Te Yao
Rok vydání: 2010
Předmět:
Zdroj: Biochemical Systematics and Ecology. 38:195-201
ISSN: 0305-1978
DOI: 10.1016/j.bse.2009.12.016
Popis: Sequences of the partial 293-bp nuclear Z-chromosome-linked chromo-helicase binding protein (CHD-Z) and 729-bp mitochondrial cytochrome b (cyt b) genes were obtained from two species of the same family Pycnonotidae of Pycnonotus sinensis (P. sinensis, Chinese Bulbul) and Pycnonotus taivanus (P. taivanus, Taiwan Bulbul) distributed in Taiwan. A panel of 10 individuals (n = 5 for each) with unknown relationship was used to characterize single nucleotide polymorphisms (SNPs) of these genes. We identified 2 and 10 SNPs in CHD-Z and cyt b loci, respectively. Frequency of SNPs was 10 per every 1465- and 729-bp on average. Pairwise nucleotide divergences of CHD-Z and cyt b genes among 10 specimens ranged from 0 to 0.0034 and 0 to 0.0055, respectively. Phylogenetic analysis suggested that the P. taivanus group assignment based on the CHD-Z and cyt b sequences is obviously very similar to P. sinensis.
Databáze: OpenAIRE