| Autor: |
Jeffrey Seow, Carl Graham, Sadie R. Hallett, Thomas Lechmere, Thomas J.A. Maguire, Isabella Huettner, Daniel Cox, Rebekah Roberts, Anele Waters, Christopher C. Ward, Christine Mant, Michael J. Pitcher, Jo Spencer, Julie Fox, Michael H. Malim, Katie J. Doores |
| Rok vydání: |
2021 |
| Předmět: |
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| Popis: |
Although the antibody response to COVID-19 vaccination has been studied extensively at the polyclonal level using immune sera, little has been reported on the antibody response at the monoclonal level. Here we isolate a panel of 44 anti-SARS-CoV-2 monoclonal antibodies (mAbs) from an individual who received two doses of the ChAdOx1 nCoV-19 (AZD1222) vaccine at a 12-week interval. We show that despite a relatively low serum neutralization titre, mAbs with potent neutralizing activity against the current SARS-CoV-2 variants of concern (B.1.1.7, P.1, B.1.351 and B.1.617.2) were obtained. The vaccine elicited neutralizing mAbs form 8 distinct competition groups and bind epitopes overlapping with neutralizing mAbs elicited following SARS-CoV-2 infection. AZD1222 elicited mAbs are more mutated than mAbs isolated from convalescent donors 1-2 months post infection. Spike reactive IgG+ B cells were still detectable 9-months post boost. These findings give molecular insights into AZD1222 elicited antibody response. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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