Fine structure and assembly pattern of a minimal myophage Pam3
| Autor: | Feng Yang, Yong-Liang Jiang, Jun-Tao Zhang, Jie Zhu, Kang Du, Rong-Cheng Yu, Zi-Lu Wei, Wen-Wen Kong, Ning Cui, Wei-Fang Li, Yuxing Chen, Qiong Li, Cong-Zhao Zhou |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Proceedings of the National Academy of Sciences. 120 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.2213727120 |
| Popis: | The myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with a rather complicated structure, the assembly pattern and tail contraction mechanism of myophage remain largely unknown. Here, we present the fine structure of a freshwater Myoviridae cyanophage Pam3, which has an icosahedral capsid of ~680 Å in diameter, connected via a three-section neck to an 840-Å-long contractile tail, ending with a three-module baseplate composed of only six protein components. This simplified baseplate consists of a central hub-spike surrounded by six wedge heterotriplexes, to which twelve tail fibers are covalently attached via disulfide bonds in alternating upward and downward configurations. In vitro reduction assays revealed a putative redox-dependent mechanism of baseplate assembly and tail sheath contraction. These findings establish a minimal myophage that might become a user-friendly chassis phage in synthetic biology. |
| Databáze: | OpenAIRE |
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