Modulation of vascular cell mobility for tissue engineering of cardiovascular implants – a 3-D approach

Autor: M Ramsperger-Gleixner, M Fittkau, T Fischlein
Rok vydání: 2010
Předmět:
Zdroj: The Thoracic and Cardiovascular Surgeon. 58
ISSN: 1439-1902
0171-6425
Popis: Purpose: Spontaneous healing of artificial cardiovascular implants in terms of a directed tissue ingrowth of vascular cells is a major goal in tissue engineering. Graft modification by cell interactive peptides might not only facilitate cell adhesion to the prosthesis, but could also direct cellular ingrowth towards a functional tissue substitute. Methods: A 3-D in vitro transmigration model (modified Boyden chamber) was applied to examine cellular mobility on peptides such as SIKVAV and RYVVLPR (laminin derived) when combined with the cell adhesive peptide RGD (derived from fibronectin). Peptides were linked to an inert polyethylene glycol (PEG) matrix, which was crosslinked by a MMP-1 cleavable peptide. Transmigration was quantified after 48h/36h (microvascular endothelial cells MVEC/smooth muscle cells SMC resp.) in normoxia and hypoxia (n=4). Results: MVEC transmigration through RGD-PEG was increased by hypoxia (cellcounts: 3.1vs7.9, p=0.008) and by adding SIKVAV to the matrix (normoxia: 3.1vs6.8, p=0.01; hypoxia: 7.9vs11.6, p=0.03). SMC transmigration through RGD-PEG was not increased by hypoxia but by adding SIKVAV to the matrix (normoxia: 55.8vs107.6, p=0.001). In RGD+SIKVAV transmigration was decreased by hypoxia (74.2vs107.6, p=0.001). In RGD+RYVVLPR both cell lines did not show any differences in transmigration speed. Conclusion: In vitro the mobility of vascular cell lines within a 3-D matrix can selectively be influenced by peptide modification of this matrix and by hypoxia. The transmigration model and our results might raise the possibility of creating preferential ingrowth matrices for use in tissue engineering of cardiovascular implants.
Databáze: OpenAIRE
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