Mitochondrial DNA Polymorphisms Revealed by RAPD Assays Distinguish the Male-sterile and Male-fertile Cytoplasms in Pearl Millet

Autor:	A. C. Chandra-Shekara, S. R. Bhat, Bhupinder Singh, Boddupalli M. Prasanna
Rok vydání:	2005
Předmět:	Genetics Mitochondrial DNA Software maintainer Plant Science Biology engineering.material Bootstrap analysis RAPD Genetic marker Plant biochemistry engineering Agronomy and Crop Science Pearl health care economics and organizations Biotechnology Hybrid
Zdroj:	Journal of Plant Biochemistry and Biotechnology. 14:21-25
ISSN:	0974-1275 0971-7811
Popis:	Diversification of cytoplamic male sterile (CMS) sources is of considerable significance in pearl millet, considering that almost all the commercially cultivated hybrids, particularly in India, are based on a single CMS source, A1. We analyzed the mitochondrial DNA (mtDNA) polymorphisms among five pearl millet CMS sources (A1 to A5) and a male-fertile maintainer (B) line, all in an isonuclear background. Analysis using 21 random primers led to identification of polymorphic bands specific to the A1, A2, A3 and A5 CMS lines. Two RAPD primers, OP-G12 and OP-G19, in combination were able to distinguish all the male-sterile and male-fertile cytoplasms. Highly effective for this purpose also were four RAPD markers, OP-B7, OP-D8, OP-F10 and OP-G12. Cluster analysis, followed by bootstrap analysis, of the mtDNA dataset revealed two distinct clusters: cluster-I comprising the A1, A2, A3 CMS lines and the male-fertile line, and cluster-II comprising the A4 and A5 CMS lines.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ea240938eaa942639620f754c9af08ea https://doi.org/10.1007/bf03263219 Zobrazit plný text záznamu Full text from SpringerLink