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The biological response to PDT depends on the photosensitizer accumulation to the malignant cells, the tumor vasculature, the inherent cell sensitivity to the photodynamic effect, etc. The in-vivo measurement of physical parameters related with these factors can serve in the understanding of the mechanisms involved in the cell death, as well as in the optimization of the treatment procedure. In this paper we demonstrate a modular diffuse reflection and fluorescence emission imaging colorimeter. We have used it for the in-vivo on-line quantitative measurement and mapping the time course fluorescence intensity after ALA application and for the tissue color changes associated with the erythema developed during irradiation. Based on the obtained quantitative data, we investigate the correlation between the photosensitizer accumulation before light irradiation and the hemodynamic changes, revealed as erythema development, occurring during light irradiation. Their relative significance as predicting factors for the photodynamic treatment effectiveness is also evaluated.© (1997) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only. |
