Presence and expression of NAD+-dependent sorbitol dehydrogenase and sorbitol-6-phosphate dehydrogenase genes in strawberry
| Autor: | Katsuhiro Shiratake, Yoshinori Kanayama, S. Duangsrisai, Kunio Yamada, Nancy Bantog, Shohei Yamaki |
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| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | The Journal of Horticultural Science and Biotechnology. 82:191-198 |
| ISSN: | 2380-4084 1462-0316 |
| Popis: | SummaryThe sorbitol metabolic pathway in strawberry (Fragaria ananassa Duch. var. ‘Nyoho’) is unclear, despite strawberry belonging to the family Rosaceae. Therefore, the presence or absence of genes for NAD+-dependent sorbitol dehydrogenase (NAD-SDH) and sorbitol-6-phosphate dehydrogenase (S6PDH) in strawberry, and their levels of expression related to growth were investigated. A full-length cDNA encoding the NAD-SDH gene in strawberry (FaSDH) was obtained using rapid amplification of cDNA ends (RACE). The full-length clone consisted of 1,399 bp and contained a 1,083 bp open reading frame encoding 361 amino acids with a calculated molecular mass of 38.8 kDa, and a predicted isoelectric point (pI) of 6.08. This nucleotide sequence showed 79 – 85% similarity with the NAD-SDH genes of other plants. Its amino acid sequence contained both zinc- and NAD-binding sites, confirming that this sequence belonged to the NAD-SDH gene family. Southern blotting suggested that the genomic copy number of FaSDH was less th... |
| Databáze: | OpenAIRE |
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