Demineralized crab and shrimp shell powder: Cost effective medium for bacillus Sp. R2 growth and chitinase production
| Autor: | Ben Amar Cheba, Ahmad Rafik EL-Mahdy, Taha I. Zaghloul |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
animal structures 02 engineering and technology Bacterial growth 01 natural sciences Industrial and Manufacturing Engineering chemistry.chemical_compound Chitin Artificial Intelligence 010608 biotechnology Food science Shellfish biology fungi food and beverages 021001 nanoscience & nanotechnology biology.organism_classification Enzyme assay Shrimp body regions chemistry Chitinase biology.protein Prawn 0210 nano-technology Bacteria |
| Zdroj: | Procedia Manufacturing. 22:413-419 |
| ISSN: | 2351-9789 |
| Popis: | Crustaceous wastes such as shrimp, crab and lobster, are chitinous solid waste of the shellfish processing industry. to select the best substrates for bacterial growth and enzyme activity, different poor and enriched media were investigated, furthermore, the non-dematerialized and dematerialized shells powders of crab, shrimp, prawn and the mixed powders of crab+ shrimp shells and crab+ prawn shells were tested as substrates for and chitinase production by the chitin degrading bacterium Bacillus sp. R2 in a submerged fermentation (SMF) culture. The results revealed that, the tested poor media failed to enhance enzyme production, whereas the tested enriched media promote growth and protein content efficiently, in contrast the demineralized chitinous wastes were generally good carbon and nitrogen sources for growth and chitinase production. The most favorable substrates were demineralized crab + prawn followed by crab + shrimp. The obtained results would certainly encourage the utilization of shellfish processing (Crab and Shrimp Shell) waste for the industrial production of chitinase via submerged fermentation (SMF). |
| Databáze: | OpenAIRE |
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