Identification of Treponema pallidum-specific protein biomarkers in syphilis patient serum using mass spectrometry

Autor:	Li-Li Liu, Hui-Lin Zhang, Hui-Min Tian, Dan Liu, Li-Rong Lin, Tian-Ci Yang, Man-Li Tong
Rok vydání:	2021
Předmět:	Microbiology (medical) Specific protein Treponema biology business.industry medicine.disease Mass spectrometry Serum samples biology.organism_classification Microbiology Immunology medicine Biomarker (medicine) Syphilis In patient business After treatment
Zdroj:	Future Microbiology. 16:1041-1051
ISSN:	1746-0921 1746-0913
Popis:	Aim: To screen novel biomarkers in serum of syphilis patients using a mass spectrometry-based method. Materials & methods: Sera were collected from 18 syphilis patients and divided into three groups. Every six serum samples (before and after treatment) in each group were pooled and detected by mass spectrometry. Results: Twenty-five unique peptides corresponding to 15 Treponema pallidum proteins were discovered. Among them, Tp0369 was discovered as a promising biomarker candidate in this study. Tp0524 and Tp0984 levels decreased 0.38-fold and 0.51-fold after BPG treatment, respectively, which may be related to disease outcomes of syphilis. Conclusion: These findings confirmed the presence of detectable T. pallidum protein in patients' serum, which could promote the development of syphilis diagnostics.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e9f3e32f2015c9739c31ecced3079a3c https://doi.org/10.2217/fmb-2021-0172 Zobrazit plný text záznamu