Isolation and particle characterization of different strains of BmNPV isolated from south Indian region

Autor: Madhuri Sathyanarayana, S.E. Neelagund, Rajat Hegde, B. Sajjan Dayanand
Rok vydání: 2022
Předmět:
Zdroj: Research Journal of Biotechnology. 17:103-110
ISSN: 2278-4535
0973-6263
DOI: 10.25303/1703rjbt103110
Popis: Grasserie is a deadly viral disease which turns a clean creamy white silkworm into zombie worm. The nuclear polyhedrosis of B. mori caused by Nuclear Polyhedrosis Virus strain Bm (BmNPV) showing jaundice-like symptoms is collectively referred to as polyhedrosis. This DNA virus multiplies only in the nucleus of the host cells forming its complete structure in the host cell. The drawback of this disease is huge amount of crop loss. And to avoid this, the only way is prevention or the use of hazardous chemicals. To avoid all these, finding a new natural way is necessary. Before we think of controlling the virus, one should understand the detail characters of BmNPV. Light scattering is a fundamental analytical technique for the characterization of particulate materials and is most commonly applied to colloidal systems, nanoparticles and acromolecules in solution or dispersion to determine particle size and zeta Potential. This examination mainly focused on size and surface charge determination using SZ-100 nanoparticle series instrument. Further, the average OB size was found to be 150.7 nm, 138.8 nm, 143.8 nm, 140.0 nm and 140.5 nm in diameter for HRH, HSR, KNK, MSI and VKK BmNPV strain respectively. The zeta potential is often measured as a function of pH or other change in the chemistry to help formulators create new products with a long shelf life. The zeta-potentials of isolates were found to be more of negative because of the presence of PEP with the charge of - 29.0 mV, -35.4 mV, -45.0 mV, -28.1 mV and -23.5 mV for HRH, HSR, KNK, MSI and VKK BmNPV strain respectively. Thus, by exposing, these aspects may help to deduce in detail structural characteristics of different south Indian strains of BmNPV.
Databáze: OpenAIRE
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