First detection of grapevine virus F in Iran
| Autor: | Simin Sabaghian, Farshad Rakhshandehroo, Toufic Elbeaino, H. R. Zamanizadeh |
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| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Journal of Plant Pathology. 100:111-112 |
| ISSN: | 2239-7264 1125-4653 |
| DOI: | 10.1007/s42161-018-0002-5 |
| Popis: | One of the most important viral diseases of grapevine is rugose wood (RW), which is a complex of graft-transmitted viruses that affect the bark and decrease vigor and the life span of infected plants (Credi 1997, Plant Dis 81:1288-1292). One foveavirus (Rupestris stem pitting-associated virus, RSPaV) and five vitiviruses (grapevine virus A [GVA], GVB, GVD and two recently discovered [GVE and GVF]) are associated with the RW complex (Nakaune et al. 2008, Arch Virol 153:1827-1832; Al Rwahnih et al. 2014, J Plant Pathol 96:219-222). However, the definitive etiology of this disease is not well established. In spring 2017, 118 grapevine leaf samples were collected from major grapevine growing regions of Alborz province (north of Iran) and assayed for the presence of GVF. Total RNA was extracted from vein tissues using a silica-based protocol and reverse-transcribed using random primers (Roche Diagnostics, Milan, Italy) and M-MLV reverse transcriptase according to the manufacturers’ instruction (LifeTechnologies, Milan, Italy). Forward (GVF-CPs: 5'-ATGGCTCAGATATCAAGAAGGATG-3') and reverse (GVF-CPa: 5'-TCAGATCTCAGCTGCTTGTTCACCG-3') primers were designed and used in RT-PCR for the amplification of the complete coat protein gene (597 bp) of GVF. Results showed that 41% (48 of 118) of the collected samples were infected with GVF. No significant association could be made between the presence of GVF and symptoms such as vein clearing, line patterns, mottling and wood necrosis seen on infected plants. Three GVF PCR-generated amplicons were purified, cloned and sequenced. BlastN analysis of the three sequences deposited in GenBank as accession numbers (MG019721, MG019723 and MG019728) showed the highest nucleotide identity (96%) with the coat protein gene of a South African GVF isolate (KP114220) and the lowest identity (95%) with an American GVF isolate (JX105428). To the best of our knowledge, this is a first report of GVF infecting grapevine in Iran. |
| Databáze: | OpenAIRE |
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