Possible correlations between alcohol motivation level and electrophysiological structure of sleep in rats
| Autor: | Krivonosov Sk, Zorin Na, A. D. Efremov, Ionova Nk, T. I. Reshetova |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
chemistry.chemical_classification
Chromatography Alpha (ethology) General Medicine Heparin Polysaccharide General Biochemistry Genetics and Molecular Biology Electrophoresis chemistry medicine Trophoblastic beta 1-Glycoprotein Pregnancy proteins Beta (finance) Pregnancy-Specific beta 1-Glycoproteins medicine.drug |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 103:442-444 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/bf00842460 |
| Popis: | It has been found that in mild acid (pH 6.6) and mild-alkaline media (pH 7.7) both pregnancy proteins form complete precipitates. In more alkaline buffer solutions the form of alpha 2-glycoprotein (alpha 2-GP) precipitate is preserved, while trophoblastic beta 1-glycoprotein (TBG) shows three immunochemically identical components with different electrophoretic mobility. The form with beta-globulins mobility predominates, and minor fragments are presented by alpha- and gamma-components. All TBG forms are clearly seen at pH 8.6. In more alkaline medium (pH 10.0) the clarity of the precipitates drastically decreases. It is shown that heparin introduction into the gel of first dimension electrophoresis increases anode electrophoretic mobility of both proteins at polysaccharide concentration of at least 0.1 mg/ml. Large amounts of heparin cause the increase in TBG alpha-component precipitate area and the decrease in the form with beta-globulins mobility. At the same time alpha 2-GP precipitate area and form remain unchanged. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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