Variation ofBurkholderia cenocepaciavirulence potential during cystic fibrosis chronic lung infection
| Autor: | Ana S. P. Moreira, Arsenio M. Fialho, Sandra C. dos Santos, Sílvia A. Sousa, Jorge H. Leitão, Ana Pinto-de-Oliveira, Christian G. Ramos, Dalila Mil-Homens, Carla P. Coutinho, Isabel Sá-Correia |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Lineage (genetic) Burkholderia cenocepacia biology 030106 microbiology Immunology Virulence medicine.disease biology.organism_classification Microbiology Cystic fibrosis Galleria mellonella Agar plate 03 medical and health sciences Chronic infection Infectious Diseases Genotype-phenotype distinction medicine Parasitology |
| Zdroj: | Virulence. 8:782-796 |
| ISSN: | 2150-5608 2150-5594 |
| Popis: | During long-term lung infection in cystic fibrosis (CF) patients, Burkholderia cenocepacia faces multiple selective pressures in this highly stressful and fluctuating environment. As a consequence, the initial infecting strain undergoes genetic changes that result in the diversification of genotypes and phenotypes. Whether this clonal expansion influences the pathogenic potential is unclear. The virulence potential of 39 sequential B. cenocepacia (recA lineage IIIA) isolates, corresponding to 3 different clones retrieved from 3 chronically infected CF patients was compared in this study using the non-mammalian infection hosts Galleria mellonella and Caenorhabditis elegans. The isolates used in this retrospective study were picked randomly from selective agar plates as part of a CF Center routine, from the onset of infection until patients' death after 3.5 and 7.5 y or the more recent isolation date after 12.5 y of chronic infection. The infection models proved useful to assess virulence potential ... |
| Databáze: | OpenAIRE |
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