Human platelet antigen4 (Br) genotyping by ASPA: allele-specific primer amplification (PCR-SSP)

Autor:	A. E. G. Kr. von dem Borne, P. M. M. Bleeker, J. Heeremans, S. Simsek
Rok vydání:	1994
Předmět:	Genetics Specific primers Genotype Human platelet Hematology Allele Biology Platelet antigen Primer (molecular biology) Molecular biology Genotyping Phenotype
Zdroj:	British Journal of Haematology. 88:659-661
ISSN:	1365-2141 0007-1048
Popis:	Summary. We have developed a PCR assay named ASPA (allele-specific primer amplification) to determine the HPA-5a and −5b genotypes. It consists of two PCR-reactions. One primer of each priomer set has a 3’-end nucleotide which is specific for A or G at position 505 of the nHPA-5b or −5a allele respectively. The HPA-5 genotypes determined in this way were strictly concordant with the genotypes established by the PCR-ASRA and with the phenotypes established using MAIPA. The ASPA is a rapid and reliable technique and can be used for the determination of alleles which code for platelet antigen allotypes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e9d019a38edc3579b2e5285111ead0fa https://doi.org/10.1111/j.1365-2141.1994.tb05096.x Zobrazit plný text záznamu Plný text