Observation of different ceramide species from crude cellular extracts by normal-phase high-performance liquid chromatography coupled to atmospheric pressure chemical ionization mass spectrometry
| Autor: | Peter D. R. Moeller, Bart-Jan Kroesen, Benjamin J. Pettus, Yusuf A. Hannun, Alicia Bielawska, Zdzislaw M. Szulc, Mark Busman |
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| Rok vydání: | 2003 |
| Předmět: |
Electrospray
Ceramide Chromatography Chemistry Organic Chemistry Analytical chemistry Atmospheric-pressure chemical ionization Mass spectrometry High-performance liquid chromatography Thin-layer chromatography Analytical Chemistry chemistry.chemical_compound Quadrupole ion trap Sphingomyelin Spectroscopy |
| Zdroj: | Rapid Communications in Mass Spectrometry. 17:1203-1211 |
| ISSN: | 0951-4198 |
| DOI: | 10.1002/rcm.1043 |
| Popis: | Normal-phase high-performance liquid chromatography (NP-HPLC) coupled to atmospheric pressure chemical ionization mass spectrometry (APCI-MS) allows qualitative analysis of endogenous ceramide and dihydroceramide species from crude lipid extracts utilizing chromatographic methods readily adaptable from commonly used thin layer chromatography (TLC) conditions. Qualitative information for the species comes from observation of differences in chromatographic and mass spectrometric behavior between species. Application to the analysis of ceramide and dihydroceramide from various cell lines is demonstrated. The results show the species profile in each cell line to be unique despite growth under identical conditions. The results from APCI-MS analysis corroborate and enhance information acquired from use of the diacylglycerol kinase assay for total ceramide measurement. This technique readily allows the previously difficult distinction between ceramide and dihydroceramide species. |
| Databáze: | OpenAIRE |
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