Optimization of Electrofusion Parameters for Producing Hybridomas Synthesizing Human Monoclonal Antibodies

Autor:	I.G. Shemyakin, O.V. Kalmantaeva, Ya.O. Romanenko, A.S. Kartseva, M.A. Marin, V.V. Firstova, I.A. Dyatlov, A. K. Ryabko, A.E. Khlyntseva
Rok vydání:	2021
Předmět:	030110 physiology 0301 basic medicine Electrofusion 03 medical and health sciences 030104 developmental biology Ecology Biochemistry medicine.drug_class Chemistry medicine Monoclonal antibody Applied Microbiology and Biotechnology Biotechnology
Zdroj:	Biotekhnologiya. 37:65-75
ISSN:	2500-2341 0234-2758
Popis:	Monoclonal antibodies are an established treatment for many diseases, including cancer, toxic conditions and infectious diseases. The goal of this work was to optimize the conditions for obtaining hybridomas secreting human monoclonal IgG antibodies. A new protocol was developed for efficient electrofusion of human B lymphocytes with partner cells. Electrofusion parameters were optimized, and the preferred partner cell line and the ratio of cells involved in the fusion were selected. Two myeloma cell lines, K6H6/B5 and SHM-D33, were tested in detail, and the highest fusion efficiency was observed for K6H6/B5. Three hybridomas that secreted fully human monoclonal IgG antibodies were obtained using an optimized protocol electrofusion; the secretion was observed within a month, which indicates the stability of the clones and the absence of chromosome segregation. hybridoma, human monoclonal antibodies, PEG, electrofusion The work was financially supported by the Ministry of Science and Higher Education of the Russian Federation (Agreement no. 075-15-2019-1671, October 31, 2019).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e9af5f89bd9d4f26bfa051976cf738a7 https://doi.org/10.21519/0234-2758-2021-37-2-65-75 Zobrazit plný text záznamu