| Popis: |
Summary A microcosm technique was used to determine the ecotoxicity of the chemical warfare agent HD (mustard) to the indigenous soil microinvertebrate communities. HD was thoroughly incorporated into Sassafras sandy loam (SSL) soil (4.9% OM) and an oak-beech forest silt loam soil (FS, 16% OM) at nominal HD concentrations ranging from 6 to 1076 mg kg−1. After a 7-day incubation period, microarthropods were extracted from soils using a high-gradient extractor and sorted to Acari suborders Prostigmata, Mesostigmata, and Oribatida, and the insect order Collembola. Nematodes were extracted using Baermann funnels and were sorted into bacterivore, herbivore, fungivore and omnivore/predator trophic groups. Microarthropods were more sensitive to HD in both soil types compared with nematodes. The EC50 values for total numbers of microarthropods in SSL and FS were similar (65 and 71 mg kg−1, respectively). The EC50 values for total numbers of nematodes were 130 and 235 mg kg−1, respectively. Toxicity of HD to nematodes was significantly greater in SSL soil compared to FS, based on 95% confidence intervals. Results show that community-level assessment of chemical toxicity in soil using a microcosm assay is sufficiently robust and can provide the means for validating the ecotoxicity data from standardized laboratory single-species toxicity tests. |
Full Text from ScienceDirect