A CAT reporter construct containing 277bp GNAT2 promoter and 214bp IRBP enhancer is specifically expressed by cone photoreceptor cells in transgenic mice
| Autor: | Wei-Bao Fong, Candace W.-C. Kao, Shao-Ling Fong, Winston W.-Y. Kao, Richard Converse, Saixia Ying |
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| Rok vydání: | 1998 |
| Předmět: | |
| Zdroj: | Current Eye Research. 17:777-782 |
| ISSN: | 1460-2202 0271-3683 |
| DOI: | 10.1080/02713689808951257 |
| Popis: | Purpose. The α-subunit of human cone transducin plays an important role in interacting with visual pigment and activating the cGMP-dependent phosphodiesterase (cGMP-PDE). The human GNAT2 gene (cone transducin α-subunit) has been cloned and characterized by Fong et al.10 In this report, we describe the use of transgenic mice to characterize the tissue specificity of the GNAT2 promoter.Methods. A chimeric reporter gene construct which consists of a 277 bp 5′-flanking fragment of the GNAT2 gene at 5′ end of the chloramphenicol acetyltransferase (CAT) gene and a 214 bp enhancer region from the human interphotoreceptor retinoid-binding protein (IRBP) gene at the 3′ end of the CAT gene was used to generate transgenic mice. Transgenic mice were identified by Southern blot hybridization and polymerase chain reaction (PCR) analysis using tail DNA from experimental animals. Immunostaining was used to study the developmental expression of CAT and the endogenous GNAT2 gene.Results. Analysis of four transgenic mouse l... |
| Databáze: | OpenAIRE |
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