| Popis: |
This chapter deals with the basic physical analytical methods and separation procedures of spectrophotometry, electrodes, chromatography, radioactivity, and electrophoresis. It talks about the simple procedures which are appropriate for laboratory classes or are routinely used in a research laboratory. Gas chromatography (GC) is used to monitor production of methane gas, an end product of the consortium growing on the phenol. Additional options for filters are offered by scintillation cocktails that dissolve cellulose acetate, nitrocellulose, and other types of filter membranes, aiding reproducibility and counting efficiency. Even though these formulations are classified as biodegradable, nonradioactive aliquots still need to be disposed of as organic hazardous waste by the proper chemical or radiation safety authorities. Gels used for separations of proteins and short oligonucleotides are usually made of polymerized and cross-linked acrylamide. A particularly effective example of the original method is described by O'Farrell, who separated 1,000 proteins from an Escherichia coli extract. Chromatographic methods have been successfully used in enzyme purification. The chapter attempts to describe analytical and purification methods most commonly used by the microbiologist. There are many other methods that rely on instrumentation that is too expensive to be found in all but a few individual labs. |
