| Popis: |
This study aims to create bone graft from porcine’s cancellous bone. Cancellous bone is one of the commonly used materials due to its natural porous structure. However, it can arise a serious of immune responses. So we decellularized it to take out all cells inside. Cancellous blocks (3x4x4 mm3) were first treated with Tris-HCl for 6 hours to remove all soft tissues. Then, they were partially demineralized in 0,6M HCl, and were further treated in a decellularizing solution (acetone or chloroform/methanol). The treated blocks were stained with hematoxylin and eosin to assess efficiency of decellularization. Umbilical cord blood-derived cells (UCBDCs) were seeded on the acellular cancellous bone and cultured for some of days to evaluate biocompatibility in vitro. The result shows that cancellous bone blocks treated by using two decellularizing solutions have none cell; the most efficiency of cell seeding into scaffold were carried out at 2000 rpm in 1 minute and repeat 4 times. After 7 days culturing in vitro, cells adherent, spread and are capable of migrating and proliferating. The adhesion and spread of UCBDCs into scaffold were imaged with SEM. In this way, we made successfully a porous scafflod from porcine’s cancellous bone and this scaffold support the cell attachment. |
