LINE Retrotransposition Assays in Saccharomyces cerevisiae
| Autor: | Axel V. Horn, Jeffrey S. Han |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Transposable element biology ved/biology 05 social sciences Saccharomyces cerevisiae ved/biology.organism_classification_rank.species food and beverages Mutagenesis (molecular biology technique) Retrotransposon Genomics Computational biology biology.organism_classification Genome Long interspersed nuclear element 03 medical and health sciences 030104 developmental biology 0502 economics and business 050211 marketing Model organism |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493933709 |
| DOI: | 10.1007/978-1-4939-3372-3_9 |
| Popis: | Long interspersed nuclear element (LINE) retrotransposons make up significant parts of mammalian genomes. They alter host genomes by direct mutagenesis through integration of new transposon copies, by mobilizing non-autonomous transposons, by changes in host gene activity due to newly integrated transposons and by recombination events between different transposon copies. As a consequence, LINEs can contribute to genetic disease. Simple model systems can be useful for the study of basic molecular and cellular biology of LINE retrotransposons. Here, we describe methods for the analysis of LINE retrotransposition in the well-established model organism Saccharomyces cerevisiae. The ability to follow retrotransposition in budding yeast opens up the possibility of performing systematic screens for evolutionarily conserved interactions between LINE retrotransposons and their host cells. |
| Databáze: | OpenAIRE |
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