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Background and Objectives Microparticles (MP) are membrane vesicles with thrombogenic and immunomodulatory properties. We determined MP subgroups from resting platelets, activated platelets and endothelial cells in donors and apheresis platelet concentrates (PC). Material and Methods MP were double stained with annexin V and CD61 (platelet-derived MP; PMP), P-selectin or CD63 (MP from activated platelets) and CD144 plus E-selectin (endothelial cell-derived MP; EMP) and detected by flow cytometry in platelet donors (n = 36) and apheresis PC (n = 11; Trima (TM)). Results PC contained MP, mainly from resting platelets [93% (90-95)], and minor fractions of PMP from activated platelets [P-selectin+ or CD63+; 4 center dot 8% (3 center dot 2-7 center dot 7) and 2 center dot 6% (2 center dot 0-4 center dot 0)]. Compared to donors, levels of annexin V+ MP, PMP, P-selectin+ and CD63+ MP were 1 center dot 7-, 2 center dot 3-, 8 center dot 6- and 3 center dot 1-fold higher in PC (all P |
