Transformation of the Plastid Genome in Tobacco Suspension Cell Cultures
| Autor: | Jeffrey M. Staub |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Homoplasmy fungi food and beverages Biology 01 natural sciences Cell biology Chloroplast 03 medical and health sciences Transformation (genetics) Tissue culture 030104 developmental biology Cell culture Subculture (biology) Plastid 010606 plant biology & botany Transformation efficiency |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071614716 |
| Popis: | Chloroplast transformation has been extremely valuable for the study of plastid biology and gene expression, but the tissue culture methodology involved can be laborious and it can take several months to obtain homoplasmic regenerated plants useful for molecular or physiological studies. In contrast, transformation of tobacco suspension cell plastids provides an easy and efficient system to rapidly evaluate the efficacy of multiple constructs prior to plant regeneration. Suspension cell cultures can be initiated from many cell types, and once established, can be maintained by subculture for more than a year with no loss of transformation efficiency. Using antibiotic selection, homoplasmy is readily achieved in uniform cell colonies useful for comparative gene expression analyses, with the added flexibility to subsequently regenerate plants for in planta studies. Plastids from suspension cells grown in the dark are similar in size and cellular morphology to those in embryogenic culture systems of monocot species, thus providing a useful model for understanding the steps leading to plastid transformation in those recalcitrant species. |
| Databáze: | OpenAIRE |
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