| Autor: |
Yong Wei, Jin Xian Ma, Hai Yun Wu, Ya Ping Yu, Yuan Liu, Yue Ming Zuo, Xiao Ying Guo |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Applied Mechanics and Materials. :111-115 |
| ISSN: |
1662-7482 |
| Popis: |
Rapid-response biosensing systems are necessary to counteract threats due to high-consequence pathogens. A disposable immunosensor suitable for quantitative determination of E.coli O157:H7 in pure cultures was investigated by electrochemical impedance spectroscopy (EIS). Electric responses of this biosensor for different cell concentrations at multi-frequencies were explored utilizing disposable screen-printed silver four-wire interdigitated microelectrodes (IMEs). Additionally, the best response frequency for the detection was studied, and the relationship between the impedance at this frequency and the concentrations of E.coli O157:H7 was established. The results showed that the impedance biosensor showed linearity from 1.15×103 CFU/mL to 1.15×106 CFU/mL at 100Hz, which yielded the model coefficient to 0.951. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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