Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducingTrichoderma reeseistrain Rut-C30
| Autor: | Sandrine Perrin, Frédérique Bidard-Michelot, Cyril Firmo, Aurelie Pirayre, Etienne Jourdier, Laurent Duval, Antoine Margeot, Corinne Blugeon |
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| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences biology 030306 microbiology Cellulase biology.organism_classification 03 medical and health sciences chemistry.chemical_compound Hydrolysis Enzyme chemistry Biochemistry biology.protein Hemicellulose Lactose Cellulose Sugar Trichoderma reesei 030304 developmental biology |
| Popis: | BackgroundThe degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process. Hydrolysis of lignocellulosic substrates is usually performed by enzymes produced and secreted by the fungusTrichoderma reesei. Studies identifying transcription factors involved in the regulation of cellulase production have been conducted but no overview of the whole regulation network is available. A transcriptomic approach with mixtures of glucose and lactose, used as a substrate for cellulase induction, was used to help us decipher missing parts in the network.ResultsExperimental results confirmed the impact of sugar mixture on the enzymatic cocktail composition. The transcriptomic study shows a temporal regulation of the main transcription factors and a lactose concentration impact on the transcriptional profile. A gene regulatory network (GRN) built using the BRANE Cut software reveals three sub-networks related toia positive correlation between lactose concentration and cellulase production,iia particular dependence of the lactose onto theβ-glucosidase regulation andiiia negative regulation of the development process and growth.ConclusionsThis work is the first investigating a transcriptomic study regarding the effects of pure and mixed carbon sources in a fed-batch mode. Our study expose a co-orchestration ofxyr1,clr2andace3for cellulase and hemicellulase induction and production, a fine regulation of theβ-glucosidase and a decrease of growth in favor of cellulase production. These conclusions provide us with potential targets for further genetic engineering leading to better cellulase-producing strains. |
| Databáze: | OpenAIRE |
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