Transient b-glucuronidase activity after infiltration of Arabidopsis thaliana by Agrobacterium tumefaciens

Autor: R. Vincenciová, M. Ondřej, Tomáš Kocábek, S. Rakouský
Rok vydání: 1997
Předmět:
Zdroj: Biologia plantarum. 39:33-41
ISSN: 1573-8264
0006-3134
Popis: Transient expression of the β-glucuronidase (GUS) gene introduced into Arabidopsis thaliana intact plants by T-DNA after vacuum infiltration of Agrobacterium tumefaciens was followed. The first incidence of GUS activity was found 2 - 3 d after treatment and a peak of activity one week after treatment in both A. thaliana races, Columbia and C24. GUS activity was sharply increased by cultivation of Arabidopsis plants at elevated temperature (29 °C) compared to cultivation at 25 °C. The density of inocula also influenced the GUS activity.
Databáze: OpenAIRE