Transient b-glucuronidase activity after infiltration of Arabidopsis thaliana by Agrobacterium tumefaciens
Autor: | R. Vincenciová, M. Ondřej, Tomáš Kocábek, S. Rakouský |
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Rok vydání: | 1997 |
Předmět: | |
Zdroj: | Biologia plantarum. 39:33-41 |
ISSN: | 1573-8264 0006-3134 |
Popis: | Transient expression of the β-glucuronidase (GUS) gene introduced into Arabidopsis thaliana intact plants by T-DNA after vacuum infiltration of Agrobacterium tumefaciens was followed. The first incidence of GUS activity was found 2 - 3 d after treatment and a peak of activity one week after treatment in both A. thaliana races, Columbia and C24. GUS activity was sharply increased by cultivation of Arabidopsis plants at elevated temperature (29 °C) compared to cultivation at 25 °C. The density of inocula also influenced the GUS activity. |
Databáze: | OpenAIRE |
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