Multifunctional Monolayer Assemblies for Reversible Direct Fluorescence Transduction of Protein−Ligand Interactions at Surfaces

Autor:	Philip D. Hampton, and Tione Buranda, Michael M. A. Sekar, Gabriel P. Lopez
Rok vydání:	1999
Předmět:	Fluorophore General Chemistry Biochemistry Combinatorial chemistry Catalysis chemistry.chemical_compound Colloid and Surface Chemistry chemistry Biotin Triethoxysilane Monolayer Organic chemistry BODIPY Ethylenedioxy Protein ligand Protein adsorption
Zdroj:	Journal of the American Chemical Society. 121:5135-5141
ISSN:	1520-5126 0002-7863
DOI:	10.1021/ja983912y
Popis:	This paper describes a convenient new method for preparing functionalizable protein-resistant monolayers that can be used to incorporate ligands and protein-sensitive fluorescent reporter groups, and the use of these monolayers for the detection of protein−ligand interactions. BODIPY X-650/665, a diode laser compatible fluorophore, and biotin, a model ligand, have been used to transduce biospecific interactions between proteins and biotin at surfaces. Silicon wafers or quartz slides were coated with (3-aminopropyl)triethoxysilane, and treated with glutaraldehyde and then 2,2‘-(ethylenedioxy)bis(ethylenediamine). The resultant surface layers are resistant to nonspecific protein adsorption and contain primary amine groups that are available for subsequent derivatization. Chemical modification of the amine-terminated monolayers thus obtained was accomplished using the N-hydroxysuccinimide active ester of BODIPY X-650/665 and biotin activated with Woodward's reagent K. Surfaces treated only with the BODIPY dy...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e939d0baf07a4377684b9a8077a32ea5 https://doi.org/10.1021/ja983912y Zobrazit plný text záznamu