Development and validation of a method for determination of PCDDs and PCDFs in human blood plasma. A multivariate comparison of blood and adipose tissue levels between Viet Nam veterans and matched controls
| Autor: | Wayne P. Wilson, Christoffer Rappe, Henry Velez, Therese Ghent-Guenther, Peter C. Kahn, Marianne Hansson, Martin Nygren, Michael Sjöström, Michael Gochfeld |
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| Rok vydání: | 1988 |
| Předmět: |
Detection limit
Multivariate statistics Environmental Engineering Chromatography Human blood Chemistry Health Toxicology and Mutagenesis Viet nam Public Health Environmental and Occupational Health Analytical chemistry Adipose tissue High resolution General Medicine General Chemistry Pollution Blood plasma Environmental Chemistry |
| Zdroj: | Chemosphere. 17:1663-1692 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/0045-6535(88)90095-1 |
| Popis: | A method which allows measurements of low levels (fg/g) of PCDDs and PCDFs in blood plasma, has been developed and validated at four fortified levels between 0.027 ppt and 0.9 ppt. The procedure involves extraction with organic solvents, multiple cleanup using various absorbents and finally detection and quantification by multiple-ion-mode high resolution gas chromato graphy/high resolution mass spectrometry. This method has been found to have a limit of detection of 0.003 to 0.02 ppt depending on the isomer studied. Using this method, samples of blood plasma have been analyzed for PCDDs and PCDFs from Viet Nam veterans from The United States, and from matched controls, and have been compared with the results of the analyses of adipose tissue. Exposed Vietnam Veterans could be separated from matched controls using a multivariate data analysis. |
| Databáze: | OpenAIRE |
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