Facile preparation of photonic and magnetic dual responsive protein imprinted nanomaterial for specific recognition of bovine hemoglobin
| Autor: | Qi Hu, Xueyan Zhen, Ruifang Ke, Xiaoyu Xie, Yusi Bu, Sicen Wang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
General Chemical Engineering
Molecularly imprinted polymer 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Combinatorial chemistry Industrial and Manufacturing Engineering Receptor–ligand kinetics 0104 chemical sciences Nanomaterials chemistry.chemical_compound Benzenesulfonic acid Molecular recognition Azobenzene chemistry Polymerization Environmental Chemistry 0210 nano-technology Selectivity |
| Zdroj: | Chemical Engineering Journal. 371:130-137 |
| ISSN: | 1385-8947 |
| DOI: | 10.1016/j.cej.2019.04.019 |
| Popis: | Stimulated response molecularly imprinted polymers (MIPs) have attracted wide attention not only because of their intriguing response to external stimuli, but also due to the desired molecular recognition ability. Herein, a novel dual responsive proteins MIPs with both photonic and magnetic stimuli has been developed by surface imprinting polymerization, where double bonds modified Fe3O4 nanoparticles, a water-soluble azobenzene containing 4-[(4-methacryloyloxy)phenylazo]benzenesulfonic acid and bovine hemoglobin (BHb) were used as magnetic core, photoswitchable functional monomer and template protein, respectively. The resultant MIPs possessed a typical spherical structure with a relative rough imprinted layer of 15 nm thickness. Moreover, the MIPs had good binding behavior toward BHb with fast binding kinetics, high adsorption capacity and satisfactory selectivity. The MIPs were proved to be well photo-controlled toward the template protein BHb under the alternating irradiation of 365 and 440 nm: 31.4% BHb was released from the MIPs at 365 nm and 67.2% BHb was bound into the MIPs at 440 nm. As a result, the MIPs were successfully applied to specifically extract template protein from actual biological sample. As a consequence, combining photocontrolled release and uptake, rapidly magnetic separation and specific recognition, the resultant MIPs with photonic and magnetic dual responsive abilities show significant advantages in the enrichment of trace protein in biological samples. |
| Databáze: | OpenAIRE |
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