Monoclonal Antibodies to Legionella pneumophila: Possible Applications in Clinical Diagnostic Tests and Epidemiologic Investigations
| Autor: | Jean R. Joly, R M McKinney, Ian D. Watkins |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
biology
medicine.drug_class Radioimmunoassay bacterial infections and mycoses biology.organism_classification Monoclonal antibody Legionella pneumophila respiratory tract diseases Latex fixation test Microbiology Antigen biology.protein medicine bacteria Sputum Antibody medicine.symptom Direct fluorescent antibody |
| DOI: | 10.1016/b978-0-12-463002-4.50012-4 |
| Popis: | Publisher Summary This chapter discusses monoclonal antibodies to Legionella pneumophila and describes its applications in clinical diagnostic tests and epidemiologic investigations. The most frequently used procedure for rapid diagnosis of legionellosis is the direct fluorescent antibody technique. This test is useful for detecting Legionella pneumophila in clinical specimens, such as lung tissue from biopsy or autopsy, peritoneal fluid, bronchial washings, sputum, and other respiratory tract specimens. The reliability of the test depends on the specificity of the fluorescein-conjugated antibodies for cell surface antigens of Legionella pneumophila and on the presence of Legionella pneumophila in sufficient concentration to allow them to be readily located by fluorescence microscopy. Other tests developed for the diagnosis of legionellosis based on the detection of soluble antigens of Legionella pneumophila in the patients' urine are radioimmunoassay, enzyme-linked immunosorbent assay, reverse passive hemagglutination test, latex agglutination test, and reverse passive agglutination test. More than one monoclonal antibody is necessary to identify the different strains of the Legionella pneumophila serogroup. |
| Databáze: | OpenAIRE |
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