A Comparative Assessment of Presence of Biological Debris And Level of Contamination on Rotary And Hand Endodontic Files Subjected To Different Cleaning Protocols Prior of SterilizationA Stereomicroscope Study
Autor: | Dhaval Bhadra, Deep J. Makati, Ruchirani P. Shah, Setu R. Bavaria, Nimisha Shah |
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Rok vydání: | 2016 |
Předmět: |
medicine.medical_specialty
business.industry Dentistry 030206 dentistry 030501 epidemiology Sterilization (microbiology) Contamination Endodontics Debris 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry Sodium hypochlorite Stereo microscope Chlorhexidine gluconate Endodontic files medicine 0305 other medical science business |
Zdroj: | IOSR Journal of Dental and Medical Sciences. 15:131-139 |
ISSN: | 2279-0853 2279-0861 |
DOI: | 10.9790/0853-150806131139 |
Popis: | To assess the presence of biological debris and level of contamination on rotary and hand endodontic files subjected to different cleaning protocols prior to sterilization using a stereomicroscope. Ninety endodontic instruments (hand and rotary file systems), after clinical use in the Department of Conservative Dentistry and Endodontics, KMSDCH, Piparia, Vadodara were used for the study. The instruments were brushed manually with a nylon brush for 10 strokes and randomly divided into five groups (3% hydrogen peroxide, 2% glutaraldehyde solution, 0.2% chlorhexidine gluconate, 3% sodium hypochlorite, ultrasonic bath with an enzymatic solution). Fifteen files directly from the manufacturer's packaging were used as control. All the instruments in the experimental groups were finally rinsed in distilled water for five minutes and dried. All the instruments were stained for 3 minutes, rinsed, dried and visualized under the stereomicroscope for presence of residual organic debris at 40X magnification. Statistical analysis was done by Kruskal-Wallis Test and Mann Whitney Test. Control group showed highest percentage of debris collection, (p value = |
Databáze: | OpenAIRE |
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