| Popis: |
Polyacrylamide gel electrophoresis was used to follow the sequential appearance within the nucleus of polypeptides synthesized in cells infected with herpes simplex virus type 1. Coelectrophoresis of 3 H-labeled uninfected cell polypeptides and 14 C-labeled nuclear polypeptides from cells pulse-labeled for 2 hr at various times after infection, showed that the synthesis of the major host polypeptides had been shut off by 4 hr post-infection. Pulse-harvesting and pulse-chase experiments revealed the presence of viral induced polypeptides in nuclei from cells labeled between 0 and 2 hr post-infection. At this early time period it was possible to detect the presence of the major structural polypeptide. Coelectrophoresis of 3 H-labeled purified viral nucleocapsids obtained by treatment of enveloped virus particles with NP-40 with 14 C-labeled nuclear extracts revealed that the majority of the nuclear polypeptides were components of the nucleocapsid. The molecular weight of the nuclear viral-induced polypeptides ranged from 11,500 to 175,000. |
