| Autor: |
Marina Arbi, Margarita Skamnelou, Spyridoula Bournaka, Sihem Zitouni, Stavroula Tsaridou, Ozge Karayel, Catherine G. Vasilopoulou, Aikaterini C. Tsika, Nikolaos N. Giakoumakis, Georgios A. Spyroulias, Matthias Mann, Mónica Bettencourt-Dias, Stavros Taraviras, Zoi Lygerou |
| Rok vydání: |
2022 |
| DOI: |
10.1101/2022.09.30.510086 |
| Popis: |
SUMMARYThe centriole duplication cycle must be tightly controlled and coordinated with the chromosome cycle. Aberrations in centriole biogenesis can lead to cancer, developmental disorders and ciliopathies. Here, we show that McIdas -previously implicated in cell cycle control and centriole amplification in multiciliated cells-is critical to maintain centriole numbers. Using expansion microscopy, we demonstrate that McIdas is present at the middle part of centrioles, where it exhibits a differential localization during the cell cycle. McIdas loss perturbs daughter centriole biogenesis and centrosomal SAS6 recruitment, whereas its overexpression induces centriole overduplication. Consistently, McIdas depletion reduces PLK4-induced centriole amplification. McIdas interacts with and is phosphorylated by PLK4 in multiple sites identified by mass spectrometry. Mutational analysis shows that McIdas phosphorylation is important for centriole number control. Overall, our results identify a novel, direct role of McIdas on centriole duplication that can link its previously characterized roles in the chromosome cycle and multiciliogenesis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
