Automated creatine kinase-MB estimation by immuno-inhibition: a clinical evaluation
| Autor: | Thomas J. Delahunty, Craig C. Foreback |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
biology
business.industry Electrophoresis technique Biochemistry (medical) Clinical Biochemistry medicine.disease Isozyme Molecular biology Creatine kinase.MB chemistry.chemical_compound chemistry Lactate dehydrogenase Creatine kinase isoenzyme biology.protein Medicine Creatine kinase Myocardial infarction business Clinical evaluation |
| Zdroj: | Clinical Chemistry. 26:568-572 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/26.5.0568 |
| Popis: | We detected the presence of creatine kinase (EC 2.7.3.2) MB isoenzyme (CK-MB) in serum by using an immunoinhibition technique to inactivate the creatine kinase isoenzyme of muscle origin (CK-MM). The GEMSAEC centrifugal analyzer is ideally suited for this procedure because it rapidly mixes reagents and has a throughput of 60 samples/h. The within-run CV was acceptable when the CK-MB value was 7.5 U/L or more. CK-MM was totally inactivated up to 5000 U/L, a value well above that usually seen in myocardial-infarct patients. We assessed the predictability of the assay for detecting myocardial infarction among 120 coronary-care patients when 2% of total CK activity was considered diagnostic and compared the results with those obtained with an electrophoresis technique. The positive predictive value of the immunoinhibition assay was 97.8%, as compared to 92% with the CK electrophoresis technique, when lactate dehydrogenase (EC 1.1.1.27) isoenzyme results were included in both cases. The optimal negative predictive value was 95.1% with the immunoinhibition assay vs 98.2% with electrophoresis. We conclude that the immunoinhibition technique for estimating CK-MB can be automated to assess myocardial status rapidly, precisely, inexpensively, and sensitively. |
| Databáze: | OpenAIRE |
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