Pseudomonas cremoris sp. nov., a novel proteolytic species isolated from cream

Autor:	Mareike Wenning, Alexandra Woller, Katharina Hofmann, Etienne V. Doll, Christopher Huptas, Siegfried Scherer
Rok vydání:	2021
Předmět:	food.ingredient biology Tributyrin Strain (chemistry) Pseudomonas Pseudomonas fluorescens General Medicine Raw milk biology.organism_classification 16S ribosomal RNA Microbiology chemistry.chemical_compound food chemistry Skimmed milk Agar Food science Ecology Evolution Behavior and Systematics
Zdroj:	International Journal of Systematic and Evolutionary Microbiology. 71
ISSN:	1466-5034 1466-5026
DOI:	10.1099/ijsem.0.004597
Popis:	During a study investigating the microbiota of raw milk and its semi-finished products, strains WS 5106T and WS 5096 were isolated from cream and skimmed milk concentrate. They could be assigned to the genus Pseudomonas by their 16S rRNA sequences, but not to any validly named species. In this work, a polyphasic approach was used to characterize the novel strains and to investigate their taxonomic status. Examinations based on the topology of core genome phylogenomy as well as average nucleotide identity (ANIm) comparisons suggested a novel Pseudomonas species within the Pseudomonas fluorescens subgroup. With pairwise ANIm values of 90.1 and 89.8 %, WS 5106T was most closely related to Pseudomonas nabeulensis CECT 9765T and Pseudomonas kairouanensis CECT 9766T. The G+C content of strain WS 5106T was 60.1 mol%. Morphologic analyses revealed Gram-stain-negative, aerobic, catalase and oxidase positive, rod-shaped and motile cells. Proteolysis on skimmed milk agar as well as lipolysis on tributyrin agar occurred at both 28 and 6 °C. Tolerated growth conditions were temperatures between 4 and 34 °C, pH values between 6.0 and 8.0, and salt concentrations of up to 5 %. Fatty acid profiles showed a pattern typical for Pseudomonas , with C16 : 0 as the dominant component. The major cellular polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol and the dominating quinone was Q-9. Based on these results, it is proposed to classify the strains as a novel species, Pseudomonas cremoris sp. nov., with WS 5106T (=DSM 111143T=LMG 31863T) as type strain and WS 5096 (=DSM 111129=LMG 31864) as an additional strain.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e89a085b009b1ea6d8819c57f1368f04 https://doi.org/10.1099/ijsem.0.004597 Zobrazit plný text záznamu