Study of enzymatic activity of microbiological biopreparation in the cultivation of cotton plant (Gossypium hirsutum L.) under saline stress conditions
| Autor: | S. S. Murodova, M. K. Khujanazarova |
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| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | IOP Conference Series: Earth and Environmental Science. 614:012125 |
| ISSN: | 1755-1315 1755-1307 |
| DOI: | 10.1088/1755-1315/614/1/012125 |
| Popis: | The changes in enzymic activity of the soils have been studied in the result of inoculation of cotton plants with microbiological preparation “Zamin-M” which was obtained on the base of association of strains Pseudomonas stutzeri SKB-308, Bacillus subtilis SKB-309 and Bacillus megaterium SKB-310 isolated from the rhizosphere of cotton plant grown in different saline soils. The results of the study showed that when the biopreparation “Zamin-M” was used, the enzymic activity of peroxidase was 1.76 ± 0.32 and in the control 1.5 ± 0.31 (mg purpurgalin/g soil, 24 hours), as well as the enzymic activity of polyphenol oxidase was 2.1 ± 0.53 and in the control 1.64 ± 0.51 (mg purpurgalin/g soil, 24 h). In the control variants, the activity of the enzyme of catalase relative to 1 g of absolutely dry soil was 1,0 ± 0,55 according to the amount of oxygen released in 5 minutes, while using the preparation its increase was observed and made 1.8±0.27 ml O2.Similar indicators were found in the study of invertase enzymic activity, when the preparation “Zamin-M” was applied, its amount constituted 271 ± 0.55 (mkg/absolutely dry soil), while in the control variant it made 111 ± 0.41 (mkg/absolutely dry soil). Urease enzymic activity was active under 24 ± 0.3 units when the preparation was used and in the control it was noted to be 14 ± 0,64 (mkg N/NH4/1 g soil) and phosphatase enzymic activity made 74±0.21 and in the control 53±0.32 (mkg P2O5/g soil, 24 hr) which led to the activation of biochemical processes in the soil. |
| Databáze: | OpenAIRE |
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