| Popis: |
Silver nanoparticles (AgNPs) have gained much attention in the treatment of dental caries due to their excellent antimicrobial activities. In this study, we have biosynthesised chitooligosaccharide coated silver nanoparticles (COS-AgNPs) using a rapid microwave-assisted method. The physical and chemical characterisations of biosynthesised COS-AgNPs were investigated using UV–visible spectroscopy (UV–vis), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), X-Ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) equipped with selected area electron diffraction (SAED). Further, the developed COS-AgNPs were assessed for antimicrobial activity on Streptococcus mutans. From the DLS analysis, the average particle size of the COS-AgNPs was found to be ∼32 nm. The morphologies of the COS-AgNPs were monodisperse and spherical in shape. XRD peaks are coherent with the standard peaks of AgNPs. Antimicrobial activity of COS-AgNPs showed a significant zone of inhibition due to the synergistic effect of COS-AgNPs. Furthermore, the antibacterial potential of developed COS-AgNPs-Ca(OH)2 paste was checked against Streptococcus mutans, Staphylococcus aureus, and Enterococcus faecalis. COS-AgNPs based composite paste showed better inhibition zone against the tested microbes. Hence, COS-AgNPs and COS-AgNPs-Ca(OH)2 based paste materials will be promising biomaterials for pulp therapy in paediatric dentistry. |
