Partial characterization of digestive tract proteinases from western corn rootworm larvae,Diabrotica virgifera

Autor:	Steven Bevilacqua, John S. Graham, Jeffrey W. Gillikin
Rok vydání:	1992
Předmět:	chemistry.chemical_classification Gel electrophoresis Larva food.ingredient biology Physiology fungi General Medicine biology.organism_classification Biochemistry Gelatin chemistry.chemical_compound Western corn rootworm Enzyme food chemistry Insect Science Cystatin Sodium dodecyl sulfate Gene
Zdroj:	Archives of Insect Biochemistry and Physiology. 19:285-298
ISSN:	1520-6327 0739-4462
DOI:	10.1002/arch.940190407
Popis:	We have partially characterized the proteolytic activity within luminal contents of the digestive tracts of larvae of the western corn rootworm, Diabrotic virgifera LeConte. At least 15 proteinases were detected based on chromatographic behavior on ion exchange high-performance liquid chromatography and their mobility on sodium dodecyl sulfate gels containing gelatin. Inhibitors of proteolytic activity indicated that these enzymes are primarily sulfhydryl proteinases. Native polycrylamide gel electrophoresis revealed that a single proteinaceous inhibitor, egg white cystatin, was capable of abolishing a substantial part of the proteolytic acitivity. Our data suggest, accordingly, that gene transfer experiments utilizing the cystatin gene may generate lines of maize which have increased resistance to western corn rootworm larvae. © 1992 Wiley-Liss, Inc.
Databáze:	OpenAIRE
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