Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis
| Autor: | Mohammadali Mohajel Shoja, Nasser Samadi, Mohammadreza Ardalan, Sepideh Zununi Vahed, Seyyedeh Mina Hejazian |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Proteinuria business.industry 030232 urology & nephrology Glomerulonephritis 030204 cardiovascular system & hematology medicine.disease Peripheral blood mononuclear cell Pathophysiology 03 medical and health sciences 0302 clinical medicine Focal segmental glomerulosclerosis Nephrology microRNA Medicine Biomarker (medicine) medicine.symptom business Nephrotic syndrome |
| Zdroj: | International Journal of Nephrology and Renovascular Disease. 13:193-201 |
| ISSN: | 1178-7058 |
| DOI: | 10.2147/ijnrd.s258624 |
| Popis: | Background Nephrotic syndrome is a common renal problem with different histopathogenesis. MicroRNAs are reported to be involved in the pathophysiology of the syndrome. The aim of this study was to study the levels of miR-30c and miR-186 in NS patients. Methods Sixty patients with primary NS (membranous glomerulonephritis (MGN, N=30) and focal segmental glomerulosclerosis (FSGS, N=30)) and 24 healthy volunteers were included. Expression levels of the miR-30c and miR-186 were evaluated in plasma and peripheral blood mononuclear cell (PBMC) samples of adult patients with NS using real-time PCR. Moreover, an in-silico analysis was performed to understand the signaling pathways and biological procedures that may be regulated by these miRNAs. Results In the MGN group, significantly elevated levels of miR-30c and miR-186 were observed in PBMC (P= 0.037) and plasma (P= 0.035) samples, respectively. Moreover, there was a significant increase in miR-30c levels in PBMC samples of the FSGS group when compared to healthy controls (P= 0.004). In ROC curve analysis, combined levels of the studied miRNAs could discriminate cases from controls in plasma and blood cells (AUC≥0.72, P Conclusion A panel of miRNAs may be potential biomarkers in plasma and PBMCs samples of NS patients with different subclasses. More investigations are needed with a large sample size to validate the diagnostic values of the reported miRNAs. |
| Databáze: | OpenAIRE |
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