Development of Tissue-Engineered Ligaments: Elastin Promotes Regeneration of the Rabbit Medial Collateral Ligament
| Autor: | Yoshiaki Suzuki, Yoshihiro Miura, Masayoshi Yamada, Hironori Unno, Takashi Horiuchi, Eri Shiratsuchi, Masaki Hirukawa, Satoshi Kageyama, Tatsuya Sato, Shingo Katayama, Masahiro Hasegawa, Keiichi Miyamoto |
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| Rok vydání: | 2017 |
| Předmět: |
Reconstructive surgery
medicine.medical_specialty medicine.medical_treatment 0206 medical engineering Biomedical Engineering Medicine (miscellaneous) Bioengineering 02 engineering and technology Biomaterials 03 medical and health sciences 0302 clinical medicine medicine 030222 orthopedics Medial collateral ligament biology business.industry Regeneration (biology) General Medicine Anatomy musculoskeletal system 020601 biomedical engineering Autotransplantation Tendon Transplantation medicine.anatomical_structure Ligament biology.protein business human activities Elastin |
| Zdroj: | Artificial Organs. 42:E102-E113 |
| ISSN: | 0160-564X |
| DOI: | 10.1111/aor.13066 |
| Popis: | When ligaments are injured, reconstructive surgery is sometimes required to restore function. Methods of reconstructive surgery include transplantation of an artificial ligament and autotransplantation of a tendon. However, these methods have limitations related to the strength of the bone-ligament insertion and biocompatibility of the transplanted tissue after surgery. Therefore, it is necessary to develop new reconstruction methods and pursue the development of artificial ligaments. Elastin is a major component of elastic fibers and ligaments. However, the role of elastin in ligament regeneration has not been described. Here, we developed a rabbit model of a medial collateral ligament (MCL) rupture and treated animal knees with exogenous elastin [100 µg/(0.5 mL·week)] for 6 or 12 weeks. Elastin treatment increased gene expression and protein content of collagen and elastin (gene expression, 6-fold and 42-fold, respectively; protein content, 1.6-fold and 1.9-fold, respectively), and also increased the elastic modulus of MCL increased with elastin treatment (2-fold) compared with the controls. Our data suggest that elastin is involved in the regeneration of damaged ligaments. |
| Databáze: | OpenAIRE |
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