Characterization of sialylated and fucosylated glycopeptides of β2-glycoprotein I by a combination of HILIC LC and MALDI MS/MS
| Autor: | Ole N. Jensen, Morten Thaysen-Andersen, Akira Kondo, Karin Hjernø |
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| Rok vydání: | 2010 |
| Předmět: |
Glycan
Chromatography Glycosylation biology Chemistry Hydrophilic interaction chromatography Proteolytic enzymes Filtration and Separation Tandem mass spectrometry Glycopeptide Analytical Chemistry carbohydrates (lipids) Matrix-assisted laser desorption/ionization chemistry.chemical_compound Biochemistry biology.protein lipids (amino acids peptides and proteins) Time-of-flight mass spectrometry |
| Zdroj: | Journal of Separation Science. 33:891-902 |
| ISSN: | 1615-9306 |
| DOI: | 10.1002/jssc.200900802 |
| Popis: | Characterization of low microgram levels of glycoprotein remains a challenge due to extensive heterogeneity of the conjugated N-glycans at each individual glycosylation site. We present an optimized, sensitive workflow for glycopeptide isolation and characterization that exploits the complementary features of RP (Poros R2) and hydrophilic (zwitter-ionic hydrophilic interaction chromatography) chromatographic resins. The glycopeptide analysis workflow was applied to human β2-glycoprotein I (β2-GPI, apolipoprotein H), which contains multiple N-glycosylation sites. Conditions for rapid proteolytic digestion of β2-GPI using low-specificity proteases were optimized to detect β2-GPI glycopeptides by MS. We demonstrate the importance of ensuring sufficient column capacity of both hydrophobic and hydrophilic stationary phases for optimal glycoprofiling by MS. The enriched glycopeptides were characterized using MALDI quadrupole TOF MS/MS. A total of 23 glycan structures, including sialylated bi- and tri-antennary complex type glycans, were characterized at three N-glycosylation sites, namely Asn-143, Asn-174 and Asn-234, of β2-GPI. Further exploration of the complementary nature of RP and HILIC stationary phases for glycopeptide isolation prior to MS analysis may eventually enable systematic analysis of complex glycoprotein samples in functional proteomic research and advance our understanding of the biological role of protein glycosylation. |
| Databáze: | OpenAIRE |
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