Simultaneous Determination of Residues of Chloramphenicol, Thiamphenicol, Florfenicol, and Florfenicol Amine in Farmed Aquatic Species by Liquid Chromatography/Mass Spectrometry
| Autor: | B Garth Burns, Jeffery M van de Riet, Melissa Christie-Fougere, Ross A Potter |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Pharmacology
Florfenicol Detection limit Residue (complex analysis) Chromatography Chloramphenicol Mass spectrometry Thiamphenicol Analytical Chemistry chemistry.chemical_compound chemistry Liquid chromatography–mass spectrometry medicine Environmental Chemistry Agronomy and Crop Science Quantitative analysis (chemistry) Food Science medicine.drug |
| Zdroj: | Journal of AOAC INTERNATIONAL. 86:510-514 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoac/86.3.510 |
| Popis: | A liquid chromatographic (LC)/mass spectrometric (MS) method was developed for determining the residues of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in a number of aquatic species. The phenicols are extracted with acetone, the extracts are partitioned with dichloromethane, the aqueous layer is removed, and the organic layer is evaporated to dryness. The residue is dissolved in dilute acid and defatted with hexane, and the aqueous layer is prepared for analysis by LC. The phenicols are determined by reversed-phase LC by using a Hypersil C18-BD column with a water–acetonitrile gradient and MS detection using selectedion recording. Calibration curves were linear for all analytes between 0.015 and 0.425 ng injected. The relative standard deviations for measurements by the proposed method were |
| Databáze: | OpenAIRE |
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