Synthesis of a new Photo-Cross-Linking Nucleoside Analogue Containing an Aryl(Trifluoromethyl)Diazirine Group: Application forEcoRII andMvaI Restriction-Modification Enzymes

Autor:	Galina A. Korshunova, Maxim G. Brevnov, O. M. Gritsenko, A. N. Topin, Elisaveta S. Gromova
Rok vydání:	1998
Předmět:	chemistry.chemical_classification Trifluoromethyl Nucleoside analogue Chemistry Stereochemistry Aryl Substrate (chemistry) Biochemistry chemistry.chemical_compound Enzyme Diazirine Genetics medicine medicine.drug
Zdroj:	Nucleosides and Nucleotides. 17:1163-1175
ISSN:	0732-8311
DOI:	10.1080/07328319808004229
Popis:	A new photo-cross-linking dU analog, 5-[4-(3-(trifluoromethyl)-3H-diazirin-3-yl)phenyl]-2′-deoxyuridine, was synthesized and incorporated into the recognition site of Eco RII and Mva I restriction-modification enzymes. The resulting base-modified 14-mer substrate was tested for cross-linking to these enzymes. Cross-linking is effected by irradiation of the enzyme-substrate complexs at 366 nm.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e864af05ec2455bdd4b8fd1b9a59e104 https://doi.org/10.1080/07328319808004229 Zobrazit plný text záznamu