Genetically engineered rice with appA gene enhanced phosphorus and minerals
| Autor: | Karabi Datta, Shinjini Sengupta, Sailendra Nath Sarkar, Aritra Karmakar, Gaurab Gangopadhyay, Sananda Bhattacharya, Swapan K. Datta |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Phytic acid Bran Phosphorus Transgene food and beverages chemistry.chemical_element Plant Science Genetically modified crops Biology Phosphate 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Germination Phytase Food science Agronomy and Crop Science 010606 plant biology & botany Biotechnology |
| Zdroj: | Journal of Plant Biochemistry and Biotechnology. 28:470-482 |
| ISSN: | 0974-1275 0971-7811 |
| Popis: | Phytic acid is the major source of phosphorus and other mineral bound compounds in many plant tissues especially seeds and bran of cereals. During germination, phytase enzyme degrades phytic acid and bound phosphate and minerals are released. The monogastric animal cannot digest phytate due to lack of the phytase enzyme. Considering that, we have generated low phytate rice by over expressing appA gene cloned from E. coli under the aleurone-specific promoter of maize zein gene. Molecular analysis confirmed the stable integration of transgene and plants were grown up to T3 generation. The T3 seeds showed around 45% decrease in seed phytate content with a fourfold increase of inorganic phosphorus (Pi) level. The enhanced iron and zinc was twofold and threefold respectively in polished seeds of transgenic plants. There was no change in germination behaviour and other morphological traits in transgenic seeds. Thus, this result provides evidence that tissue-specific expression of bacterial phytase can lead to the reduction of phytic acid in rice seeds without hampering its other physiological processes and phenotypic cost. |
| Databáze: | OpenAIRE |
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