Determination of amino acids using o-phthalaldehyde-2-mercaptoethanol derivatization effect of reaction conditions

Autor:	E. C. Beuvery, G. Wieten, R. C. Dorresteijn, G. Zomer, C.D. de Gooijer, L.G. Berwald
Rok vydání:	1996
Předmět:	chemistry.chemical_classification Reproducibility Chromatography Chemistry Organic Chemistry Concentration effect General Medicine Biochemistry Analytical Chemistry Amino acid Solvent O-Phthalaldehyde chemistry.chemical_compound Derivatization Quantitative analysis (chemistry) 2-Mercaptoethanol
Zdroj:	Journal of Chromatography A. 724:159-167
ISSN:	0021-9673
Popis:	This paper presents a systematic approach to determine the optimal reaction conditions, with respect to accuracy and sensitivity, of the quantitative determination of primary amino acids in a single run, using o -phthalaldehyde-2-mercaptoethanol derivatization. To this end an experiment was designed in which the effects of reaction time, concentration of 2-mercaptoethanol and type of solvent were determined simultaneously. The response of all parameters tested was found to be interrelated: the effect of a change in one reaction condition also depended on the other reaction conditions. The reaction conditions determined in this research resulted in an accuracy better than 0.25 μM , an average reproducibility of 0.6% and an average sensitivity of 136 fmol.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e8508e4e31d537bfbedd4974c6ec29c8 https://doi.org/10.1016/0021-9673(95)00927-2 Zobrazit plný text záznamu Full Text from ScienceDirect